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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
01/11/2013 |
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Data da última atualização: |
01/11/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GERALDINE, A. M.; LOPES, F. A. C.; CARVALHO, D. D. C.; BARBOSA, E. T.; RODRIGUES, A. R.; BRANDÃO, R. S.; ULHOA, C. J.; LOBO JUNIOR, M. |
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Afiliação: |
ALAERSON MAIA GERALDINE, UFG; FABYANO ALVARES CARDOSO LOPES, UFG; DANIEL DIEGO COSTA CARVALHO, UNB; ELDER TADEU BARBOSA, CNPAF; AMANDA RAFAELA RODRIGUES, UFG; RENATA SILVA BRANDÃO, UFG; CIRANO JOSÉ ULHOA, UFG; MURILLO LOBO JUNIOR, CNPAF. |
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Título: |
Cell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mold by Trichoderma spp. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Biological Control, Orlando, v. 67, n. 3, p. 308-316, Dec. 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.biocontrol.2013.09.013 |
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Idioma: |
Inglês |
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Conteúdo: |
Field outcomes of 10 Trichoderma spp. isolates against white mold (Sclerotinia sclerotiorum) on common beans were matched to laboratory results, to identify the causes of variance related to biocontrol effectiveness. Laboratory assays estimated sclerotia parasitism and production of the cell wall-degrading enzymes (CWDEs) lipase, NAGase, b-1,3-glucanase, b-glucosidase and protease. Field trials were carried out in 2009 and 2010 under a randomized block design and sprinkling irrigation, where 2 x 1012 spores mL -1 of each antagonist were applied to the plots at the early R5 stage. The density of S. sclerotiorum apothecia m -2 and disease severity were assessed, respectively at R7 and R8 stages, with yield and its components also estimated for each year. Field results were analyzed jointly by the Tukey?Kramer multiple comparison test, and all variables from both field and laboratory experiments were subjected to principal component analysis (PCA). In both years, isolates TR696 and TR356 of Trichoderma asperellum were effective in reducing apothecia density and disease severity. Biocontrol increased the number of pods per plant and yields up to 40% when compared to controls, even under higher disease pressure in 2010. PCA demonstrated in 2009 and 2010 that apothecia density, disease severity, NAGase, b-1,3-glucanase and number of pods were the main sources of the first component of variance. Such results suggest that the CWDEs NAGase and b-1,3-glucanase and sclerotia parasitism are key components of Trichoderma spp. action in biocontrol of S. sclerotiorum in the field, and may be used as markers to hasten the selection of new, promising isolates. MenosField outcomes of 10 Trichoderma spp. isolates against white mold (Sclerotinia sclerotiorum) on common beans were matched to laboratory results, to identify the causes of variance related to biocontrol effectiveness. Laboratory assays estimated sclerotia parasitism and production of the cell wall-degrading enzymes (CWDEs) lipase, NAGase, b-1,3-glucanase, b-glucosidase and protease. Field trials were carried out in 2009 and 2010 under a randomized block design and sprinkling irrigation, where 2 x 1012 spores mL -1 of each antagonist were applied to the plots at the early R5 stage. The density of S. sclerotiorum apothecia m -2 and disease severity were assessed, respectively at R7 and R8 stages, with yield and its components also estimated for each year. Field results were analyzed jointly by the Tukey?Kramer multiple comparison test, and all variables from both field and laboratory experiments were subjected to principal component analysis (PCA). In both years, isolates TR696 and TR356 of Trichoderma asperellum were effective in reducing apothecia density and disease severity. Biocontrol increased the number of pods per plant and yields up to 40% when compared to controls, even under higher disease pressure in 2010. PCA demonstrated in 2009 and 2010 that apothecia density, disease severity, NAGase, b-1,3-glucanase and number of pods were the main sources of the first component of variance. Such results suggest that the CWDEs NAGase and b-1,3-glucanase and sclerotia parasitism... Mostrar Tudo |
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Thesagro: |
Feijão; Fungo; Phaseolus vulgaris; Sclerotinia sclerotiorum; Trichoderma. |
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Thesaurus Nal: |
Biomarkers. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02583naa a2200289 a 4500
001 1970111
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008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.biocontrol.2013.09.013$2DOI
100 1 $aGERALDINE, A. M.
245 $aCell wall-degrading enzymes and parasitism of sclerotia are key factors on field biocontrol of white mold by Trichoderma spp.$h[electronic resource]
260 $c2013
520 $aField outcomes of 10 Trichoderma spp. isolates against white mold (Sclerotinia sclerotiorum) on common beans were matched to laboratory results, to identify the causes of variance related to biocontrol effectiveness. Laboratory assays estimated sclerotia parasitism and production of the cell wall-degrading enzymes (CWDEs) lipase, NAGase, b-1,3-glucanase, b-glucosidase and protease. Field trials were carried out in 2009 and 2010 under a randomized block design and sprinkling irrigation, where 2 x 1012 spores mL -1 of each antagonist were applied to the plots at the early R5 stage. The density of S. sclerotiorum apothecia m -2 and disease severity were assessed, respectively at R7 and R8 stages, with yield and its components also estimated for each year. Field results were analyzed jointly by the Tukey?Kramer multiple comparison test, and all variables from both field and laboratory experiments were subjected to principal component analysis (PCA). In both years, isolates TR696 and TR356 of Trichoderma asperellum were effective in reducing apothecia density and disease severity. Biocontrol increased the number of pods per plant and yields up to 40% when compared to controls, even under higher disease pressure in 2010. PCA demonstrated in 2009 and 2010 that apothecia density, disease severity, NAGase, b-1,3-glucanase and number of pods were the main sources of the first component of variance. Such results suggest that the CWDEs NAGase and b-1,3-glucanase and sclerotia parasitism are key components of Trichoderma spp. action in biocontrol of S. sclerotiorum in the field, and may be used as markers to hasten the selection of new, promising isolates.
650 $aBiomarkers
650 $aFeijão
650 $aFungo
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
650 $aTrichoderma
700 1 $aLOPES, F. A. C.
700 1 $aCARVALHO, D. D. C.
700 1 $aBARBOSA, E. T.
700 1 $aRODRIGUES, A. R.
700 1 $aBRANDÃO, R. S.
700 1 $aULHOA, C. J.
700 1 $aLOBO JUNIOR, M.
773 $tBiological Control, Orlando$gv. 67, n. 3, p. 308-316, Dec. 2013.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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